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1. Effects of Amine Fluoride on Biofilm Growth and Salivary Pellicles
2. alternative to bisphophonates currently being used in treating and preventing osteoporosis
Effects of Amine Fluoride on Biofilm Growth and Salivary Pellicles
H.C. van der Mei, E. Engels, J. de Vries, H.J. Busscher
The amine fluoride (AmF) N'-octadecyltrimethylendiamine-N,N,N'-tris
(2-ethanol)-dihydrofluoride is a cationic antimicrobial which can
have beneficial effects on plaque formation. Here, we determine
changes in pellicle and bacterial cell surface properties of the
strains Actinomyces naeslundii HM1, Streptococcus mutans NS,
S.mutans ATCC 700610, S. sobrinus HG1025 and S. oralis HM1 upon
adsorption of this AmF and accompanying effects on bacterial
adhesion and biofilm growth. In vitro pellicles had a zeta potential
of -12 mV that became less negative upon adsorption of AmF. The
chemical functionalities in which carbon and oxygen were involved
changed after AmF adsorption and AmF-treated pellicles had a greater
surface roughness than untreated pellicles. Water contact angles in
vitro decreased from 56 to 45° upon AmF treatment, which
corresponded with water contact angles (44°) measured intraorally on
the front incisors of volunteers immediately after using an AmF-
containing toothpaste. All bacterial strains were negatively charged
and their isoelectric points (IEP) increased upon AmF adsorption.
Minimal inhibitory concentrations were smallest for strains
exhibiting the largest increase in IEP. Adhesion to salivary
pellicles and biofilm growth of the mutans streptococcal strains
were significantly reduced after AmF treatment, but not of A.
naeslundii or S. oralis. However, regardless of the strain involved,
biofilm viability decreased significantly after AmF treatment. The
electrostatic interaction between cationic AmF and negatively
charged bacterial cell surfaces is pivotal in establishing reduced
biofilm formation by AmF through a combination of effects on initial
adhesion and killing. The major effect of AmF treatment, however,
was a reduction brought about in biofilm viability.
Caries Res 2008;42:19-27
A new study by researchers at the University of Southern California
(USC) School of Dentistry indicates that aspirin offers a potential
alternative to bisphophonates currently being used in treating and
In their mouse study, an aspirin regimen appeared to help the mice
recover from osteoporosis, according to Songtao Shi, D.D.S., Ph.D.,
and Takayoshi Yamaza, D.D.S., Ph.D., of the USC School of
Dentistry's Center for Craniofacial Molecular Biology. They found
that the drug seemed to prevent both improper bone resorption and
the death of bone-forming stem cells.
An aspirin regimen has been linked in earlier epidemiological
studies to better bone mineral density, but the mechanisms of its
interactions in regards to bone health had not yet been studied
extensively, Shi said.
The study appears in PLoS One (July 9, 2008, 3(7): e2615).