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Dental India update dated 24th September 2008
All dental related Google/Yahoo searches landing in

Scanning Electron Microscopy Study Confirms Effectiveness of Perio  Protect Method

D.C. Keller, Perio Protect LLC, St. Louis, MO, USA;
B. Costerton, USC School of Dentistry, Los Angeles, CA, USA;
C. Schaudinn, USC School of Dentistry, Los Angeles, CA, USA;
P. Sedghizadeh, USC School of Dentistry, Los Angeles, CA, USA;
G.S. Keller, Keller Professional Group P.C., St. Louis, MO, USA

Perio Protect LLC is consulting with one of the world’s leading microbiologists to use scanning
electron microscopy (SEM) to complete cellular evaluations to determine the effects of the
Perio Protect Method on the infection causing subgingival biofilm.

METHOD: Flexible polycarbonate carriers were placed into two 6 mm pockets and one 5mm pocket for
2 days allowing the subgingival biofilm to colonize the carrier. Carriers were inserted before and
during treatment using the Perio Tray with Perio Gel and Sumycin and subsequently examined by SEM
at different time intervals and bacterial counts were completed (Schaudinn and Costerton).

RESULTS: Cell analysis before treatment with the Perio Protect system determined the pocket ecosystem
consisted of large numbers of fusiform-like bacteria, cocci-like, short rods and in one pocket,
treponema-like. After two days using the Perio Tray most of the fusiform bacteria and treponema had
disappeared. After 17 days there were less than .02% of the periopathogenic bacteria (99.98: kill) and
what remained were some cocci-like and pleomorphic rods that were visible next to large numbers of
eukaryotic cells.

CONCLUSION: In this clinical case, the direct application of medication using the Perio Tray radically
modifies the microbial ecosystem of periodontal pockets, so that only a few bacteria species and
bacteria cells are capable of forming small colonies. SEM cell counts at 17 days determined that
greater than 99.98% of the periopathogens were eradicated. The study confirms that the Perio
Protect Method is effective in managing the microbial challenge by killing the periopathogens.

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