| What is PCR? |
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| What are three methods to use in growth and assay of viruses? |
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| What is the plaque assay used for? |
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| T or F: All viruses produce Cytopathic effect (CPE) |
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| What definition: The characteristic morphological changes which occur
in the cell due to the infection of a particular virus. |
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| T or F: Growth and assay of viruses requires a high multiplicity of
infection (MOI) |
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| The reproductive cycles of ALL viruses exhibit what common feature?
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| What is the singlemost important thing produced by viruses that gives
them the ability to multiply and determines the fate of the infected cell?
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| What type of infection: The infection and subsequent lysis of
susceptible cells by a virus. |
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| What type of infection: The abnormal growth of cells resulting from
the continuous expression of one or more viral genes. |
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| What type of infection: The virus persists in the cell in a
noninfectious form. |
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| What type of infection: The infection of a cell which does NOT result
in the multiplication of infectious viral progeny. |
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| What type of infection: The infection of a receptive cell with a virus
particle which results in the MULTIPLICATION of infectious viral progeny.
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| Name the 5 types of Infections: |
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| What determines host range? (2 things) |
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| T or F: Sometimes the target cells are at the portal of entry. |
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| What are the cells called that are targeted by the virus and result in
clinical disease? |
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| Cells that become immediately infected are susceptible cells at the
______ ___ __________. |
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| What definition? The capacity of a cell or animal to become infected.
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| What definition? The spectrum of cells which can be productively
infected by a given virus. |
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| What definition? The kinds of tissue cells and animal species that a
virus can productively infect. |
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| Steps to multiply a virus |
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| No clearly identifiable capsid. But several coats around the nucleic
acid. An example is _______ |
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| Enveloped virus w/ helical symmetry, the _____ is coiled w/in the
________. An example is ______ |
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| Enveloped virus w/ icosahedral symmetry, the _____ is surrounded by
the ________. An example is ______ |
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| Morphological subunits that can be seen when capsid is broken (hard to
"see" in an intact virus). Surface structures composed of 5-6 protein
molecules |
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| Symmetry observed w/ spherically shaped viruses. Involves the packing
together of many identical subunits. (soccer ball) |
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| Can helical particles form w/o the genome? |
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| Helical symmetry examples (5) |
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| The capsid of a virus composed of many copies of a single kind of
protein subunit arranged in a close-packed helix |
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| The simplest viral symmetry (proteins bind to nucleic acid not a
capsule) |
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| The symmetry observed w/ cylindrical viruses (like a slinky) |
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| The structure of the capsid &/or envelope (spikes) has important
implications for (3 things) |
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| Virion structural protein which locates on the cytoplasmic side of the
transmem glycoprotein spike. It attracts the completed nucleocapsid for
mem fusion. |
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| Protein or glycoprotein structure(s) which emanates from the surface
of the virus particle |
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| The lipid bilayer & associated glycoproteins that surround many
types of virus particles |
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| The capsid & nucleocapsid are composed of ___ or at most ___
different kinds of proteins in a crystalline array. Why? |
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| The complete protein-nucleic acid complex that is the packaged from of
the genome in a virus particle |
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| The protein shell directly surrounding the viral nucleic acid. |
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| The association of core proteins & the genome is required to
_______ the viral genome during viral ______ & virion _______ |
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| The _____ is the association between nucleic acid & basic proteins
either coded by the virus or histones from the host cell |
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| Retroviruses have a ______ genome |
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| The viral genome can have 3 basic morphologies |
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| The viral genome can be ______ or ______ stranded |
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| The ability of viruses to pass through ______ is used to ID an unknown
infectious agent as a bact or virus. |
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| The largest virus |
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| The smallest virus |
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| viruses are _____-_____ times smaller then the cells they infect |
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| A virus is an __________ parasite |
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| One (complete) virus particle |
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| A virus is made up of ________ & _________ |
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